2'-Amino Modified Oligonucleotides
We also offer 2'-amino modified
oligonucleotides through the use of our SUC/MOX precursor strategy. By using
this methodology we can incorporate amino-modifiers internally (currently at
pyrimidine nucleosides - C and U analogs) within the oligonucleotide. The length and
physical properties of the tether can be chosen from a wide selection of primary
aliphatic diamines to meet your particular
requirements.
Applications:
RNA folding kinetics
Our 2'-amino modified oligos can be used to internally
tether a fluorescent chromophore in order to monitor RNA tertiary folding (Silverman and Cech, Biochemistry, 1999, 38, 14224). In this study it was shown that the length of the tether to the chromophore affected the observed florescence and that the optimum tether length depended strongly on the particular site of labeling.
Facile post-synthetic modifications
Our 2'-SUC-2'-dU/-dC and 2'-MOX-2'-dU precursor
oligonucleotides allow for easy modifications directly with primary
aliphatic amines. Other post-synthetic techniques require activation prior
to modification to generate the oligonucleotide conjugate (Beban
and Miller, Bioconj. Chem., 2000, 11, 599). Our
post-synthetic manipulations using SUC and MOX precursors are all one-step
transformations to the desired modified oligonucleotide.
CONTACT INFO
301-527-0804 (tel)
301-527-8250 (fax)
fsi1@fidelitysystems.com
FOR RESEARCH USE ONLY