Fimers are primers with proprietary chemical modifications that inhibit unwanted reactions during cycle sequencing (e.g., primer-dimer artifacts and non-specific PCR). Specially designed Fimers have been routinely and successfully used for high throughput sequencing of difficult templates and highly sensitive reactions from minimal amount of templates (Polushin et al., Nucleosides Nucleotides Nucleic Acids, 2001 20, 507-14).
An example where non-specific PCR is eliminated with the use of Fimers.
Sequencing traces obtained with the use of a 35 base-long modified (A) and non-modified (B) primer.
Fimers in combination with ThermoFidelase 2 have been used for high throughput direct genomic DNA sequencing (PNAS).
FOR RESEARCH USE ONLY